A rapid, quantitative assay for titration of bovine virus diarrhoea-mucosal disease virus

The occurrence of bovine virus diarrhoea/muscosal disease in Iran

Bovine virus diarrhoea-mucosal disease and border disease in Egypt.

In Egypt, bovine virus diarrhoea-mucosal disease (BVD-MD) was initially detected as the result of a serological survey of cattle and sheep, using the serum neutralisation test. In 1970, the causal pestivirus was isolated from bovine calves and buffalo calves with pneumonia and enteritis. Mixed viral infections were also prevalent. Cases of immune tolerance were identified. The Egyptian industry...

The diagnosis of bovine virus diarrhoea-mucosal disease in cattle.

Laboratory diagnostic procedures for bovine virus diarrhoea and mucosal disease are reviewed. For serology, emphasis is placed on the virus neutralisation test and enzyme immunoassays. The techniques and the pitfalls which may be encountered in culture procedures for isolation of the virus are described, with special reference to the use of immunofluorescence and immunoperoxidase methods for th...

Glycoproteins of bovine viral diarrhoea-mucosal disease virus in infected bovine cells.

Bovine cell cultures infected with bovine viral diarrhoea-mucosal disease (BVD) virus were radiolabelled with L-[35S]methionine or D-[2-3H]mannose followed by analysis of the labelled polypeptides by radioimmunoprecipitation and polyacrylamide gel electrophoresis in one and two dimensions. Six glycoproteins were detected in infected cells. Two abundant species had Mr of 48K and 56K to 58K while...

Epidemiology of bovine virus diarrhoea virus.

A better understanding of the epidemiology and pathogenesis of bovine virus diarrhoea virus (BVDV) has emerged in recent years. Fetal infections and in particular those resulting in birth of persistently infected calves are of central importance for the epidemiology of BVDV. A prevalence of persistently infected, viraemic animals of about 1% is found in Denmark and elsewhere by examination of r...